Investigation of the polymorphism of the myostatin gene by using PCR-RFLP in two tilapia species (Oreochromis aureus and Oreochromis niloticus)

Author: Makbule Baylan 1*, Gamze Mazi 1, Numan Ozcan 2, Bahri Devrim Ozcan 3 and Ali İrfan Güzel 4
Received 14 January 2015, accepted 30 March 2015.

The aim of the study was to investigate the restriction fragment length polymorphism (RFLP) of the exon 1–3 fragments from the myostatin gene in two tilapia species, Oreochromis aureus and Oreochromis niloticus. Tissue samples were collected from a total of 50 fish, and the total genomic DNA was extracted and used for PCR amplification. Particular regions of exon 1–3 from the myostatin gene (390, 435 and 342 bp, respectively) were amplified with specific primers for each exon region by using PCR. The PCR-amplified products of each exon region were digested with appropriate restriction endonuclease enzymes and analysed using agarose gel electrophoresis.

Journal: Food, Agriculture and Environment (JFAE)
Online ISSN: 1459-0263Year: 2015, Vol. 13, Issue 2, pages 228-231.Publisher: WFL.

Article Purchasing
Impact Factor

If you like to purchase this specific document  such as article, review or this journal issue, contact us.

Specify the title of the article or review, issue, number, volume and date of the publication.

Software and compilation, Science & Technology,  all rights reserved.

Your use of this website details or service is governed by terms of use.

Authors are invited to check from time to time news or information, read more>>>

Note to Users

The requested document is freely available only to subscribers/registered users with an online subscription to the Journal of Food, Agriculture & Environment. If you have set up a personal subscription to this title please enter your user name and password. All abstracts are available for free.