In vitro propagation, callus induction, and evaluation of active compounds on Ruta graveolens

Author: Zakaria I. Al-Ajlouni 1*, Shifaa Abbas 1, Mohamad Shatnawi 2 and Ibrahim Al-Makhadmeh 1
Received 30 December 2014, accepted 28 March 2015.
Abstract

Ruta graveolens belongs to Rutaceae family. It is a medicinal plant native to Jordan and is classified as an aromatic plant. R. graveolens plant is at risk of extinction, due to over-collection, overgrazing and deforestation. Therefore, this study was conducted to investigate factors affecting R. graveolens in vitro propagation. Callus induction was conducted from leaf discs on MS (Murashige and Skoog) media supplemented with 4.0 mg/l NAA. Callus tissue was maintained by frequent subculture on MS media supplemented with 1.0 mg/l 2,4-D. Rapid multiplication of R. graveolens was achieved by culturing microshoot on MS medium containing TDZ, 2-iP, zeatin or GA3 at various concentration. Zeatin at 0.5 mg/l resulted in the highest shoot number and length. Crude extracts of callus, in vitro and ex vitro of R. graveolens were prepared by different solvents like methanol and ethanol. High performance liquid chromatography (HPLC) technique was used for quantitative analysis of five active compounds in three culture types. In vitro culture extracted by ethanol was contained the maximum content of metabolites compared to callus and ex vitro cultures.

Journal: Food, Agriculture and Environment (JFAE)
Online ISSN: 1459-0263Year: 2015, Vol. 13, Issue 2, pages 101-106.Publisher: WFL.


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