The biodegradation of wood constituents is currently understood as a multienzymatic process mediated by small molecules. Many cellulolytic and ligninolytic fungi use hydrolytic enzymes that enable them to produce monosaccharides from all of the polysaccharide components of wood. However, when these polysaccharides occur in complexes with lignin, they are resistant to hydrolytic breakdown. Currently, Ceriporiopsis subvermispora, one of the most investigated white rot fungi, is heavily used for pretreatment due to its selectively for lignin biodegradation and very low cellulose loss. Thus, the differentially expressed genes of Ceriporiopsis subvermispora were identified and studied on Pinus taeda and Eucalytus grandis cultures from 5 to 60 days. Using the differential display reverse transcription PCR technique (DDRT-PCR), a total of 106 differentially expressed cDNA fragments were identified from mycelia cultures on P. taeda. Among these fragments, 65 differential bands were sequenced and identified, and 34 sequences exhibited homologies with known proteins, such as oxidases, dehydrogenases and oxidoreductases. Three of these sequences exhibited similarities to oxalate oxidase, cellobiohydrolase and manganese superoxide dismutase, which are associated with wood biodegradation genes. The q-PCR analysis revealed that the expressions of these genes were associated with the ligninolytic and cellulolytic activities depending on the wood species that was used for fungal growth. These results might provide clues to further our understanding of fungal wood degradation abilities. Although the mechanism remains uncertain, this study identified a set of potentially important genes, including those that hypothetically encode proteins, whose differential expressions provided information that could elucidate the fungal delignification process for biotechnological applications.
Online ISSN: 1459-0263Year: 2015, Vol. 13, Issue 2, pages 232-237.Publisher: WFL.
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