In this study, the cytotoxic and genotoxic effects of aflatoxin extracted from Aspergillus niger grown on stored coffee bean seeds were investigated. hree concentrations were prepared (0.5%v,1%v,2%v) and the effects were studied after the root tips of Vicia faba were treated for three different periods of time (6, 12 and 24 hours). Gamma radiation was used as a protective agent against afltoxin mitodepressive effect; three different dosages were used (3, 5 and 10 kGy) and compared against untreated aflatoxin and negative treatment (control). Treatment with 1%v and 2%v decreased the mitotic index and the effect was significant at 0.05, while the lowest concentration (0.5%v) increased the mitotic index. In addition aflatoxin increased chromosomal aberrations, treatment with 1%v showed significant effect at 0.05. The type of chromosomes abnormalities were disturbance, sticky chromosomes during metaphase and anaphase, star metaphase, c-metaphase and tripolar in anaphase, lagging chromosomes and micronuclei. The treated aflatoxin with different dosages of gamma radiation showed less effect on mitotic index and chromosomal aberration frequency compared with untreated aflatoxin and control, gamma radiation became more effective on aflatoxin with the increase of dosage. This study encourages using gamma radiation as a protective agent against aflatoxin mitodepretion effect on cell division.
Online ISSN: 1459-0263Year: 2015, Vol. 13, Issue 2, pages 16-23.Publisher: WFL.
If you like to purchase this specific document such as article, review or this journal issue, contact us.
Specify the title of the article or review, issue, number, volume and date of the publication.
Software and compilation, Science & Technology, all rights reserved.
Authors are invited to check from time to time news or information, read more>>>
Note to Users